• DRAMP ID

    • DRAMP02036
    • Peptide Name

    • Brevinin-2-RN1 (Frogs, amphibians, animals)
    • Source

    • Rana nigrovittata
    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)
    • Gene

    • Not found
    • Sequence

    • GAFGNFLKGVAKKAGLKILSIAQCKLSGTC
    • Sequence Length

    • 30
    • UniProt Entry

    • No entry found
    • Protein Existence

    • Not found
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
    • Target Organism

      • Gram-positive bacteria: Staphylococcus aureus ATCC2592 (MIC=3.10 µg/ml), Staphylococcus aureus ATCC43300 (MIC=3.10 µg/ml), Bacillus subtilis (MIC=12.5 µg/ml);
      • Gram-negative bacteria: Escherichia coli ML-35P (MIC=25 µg/ml), Pseudomonas aeruginosa PA01 (MIC=12.5 µg/ml), Pseudomonas aeruginosa ATCC27853 (MIC=25 µg/ml).
      • Yeast: Candida albicans ATCC2002 (MIC=6.25 µg/ml).
    • Hemolytic Activity

      • [Ref.21171145] It has 17% hemolysis at 100 µg/ml against rabbit red blood cell
    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Cyclic
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Cyclization (Cys24 and Cys30)
    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys24 and Cys30.
    • Stereochemistry

    • L
    • Structure

    • Not found
    • Structure Description

    • Not found
    • Helical Wheel Diagram

    • DRAMP02036 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • Formula

    • C136H231N37O36S2
    • Absent Amino Acids

    • DEHMPRWY
    • Common Amino Acids

    • GK
    • Mass

    • 3024.68
    • PI

    • 9.9
    • Basic Residues

    • 5
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 13
    • Net Charge

    • +5
    • Boman Index

    • 4.72
    • Hydrophobicity

    • 0.513
    • Aliphatic Index

    • 101
    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 125
    • Absorbance 280nm

    • 4.31
    • Polar Residues

    • 11

DRAMP02036

DRAMP02036 chydropathy plot
    • Brevinin-2-RN1 and -RN2 could induce 17 and 21% Rabbit red blood cell hemolysis, respectively.

  • ·Literature 1
    • Title

    • Two novel antimicrobial peptides from skin secretions of the frog, Rana nigrovittata.
    • Reference

    • J Pept Sci. 2011 Jan;17(1):68-72.
    • Author

    • Liu X, Liu R, Wei L, Yang H, Zhang K, Liu J, Lai R.