General Information

    • DRAMP ID

    • DRAMP02114

    • Peptide Name

    • Raniseptin-1 (Rsp-1; Frogs, amphibians, animals)

    • Source

    • Hypsiboas raniceps (Chaco tree frog) (Hyla roeschmanni)

    • Family

    • Belongs to the frog skin active peptide family (Dermaseptin subfamily)

    • Gene

    • Not found

    • Sequence

    • AWLDKLKSLGKVVGKVALGVAQNYLNPQQ

    • Sequence Length

    • 29

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.18976634]Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=5 µM), Pseudomonas aeruginosa ATCC 27853 (MIC=10 µM), Xanthomonas citri (MIC<2 µM);
      • Gram-positive bacterium: Staphylococcus aureus ATCC 29313 (MIC=20 µM).

    • Hemolytic Activity

      • [Ref.18976634]20% hemolytic activity at 80 μM against human red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02114 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C144H237N39O39

    • Absent Amino Acids

    • CEFHIMRT

    • Common Amino Acids

    • L

    • Mass

    • 3138.7

    • PI

    • 9.83

    • Basic Residues

    • 4

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +3

    • Boman Index

    • -13.02

    • Hydrophobicity

    • -0.041

    • Aliphatic Index

    • 117.59

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 6990

    • Absorbance 280nm

    • 249.64

    • Polar Residues

    • 7

DRAMP02114

DRAMP02114 chydropathy plot

Comments Information

    • Function

    • Has antibacterial activity against Gram-negative as well as Gram-positive bacteria. And has hemolytic activity against human erythrocytes.

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Post-secretory events alter the peptide content of the skin secretion of Hypsiboas raniceps.

    • Reference

    • Biochem Biophys Res Commun. 2008 Dec 26;377(4):1057-1061.

    • Author

    • Magalhães BS, Melo JA, Leite JR, Silva LP, Prates MV, Vinecky F, Barbosa EA, Verly RM, Mehta A, Nicoli JR, Bemquerer MP, Andrade AC, Bloch C Jr.