General Information

    • DRAMP ID

    • DRAMP02219

    • Peptide Name

    • Ranatuerin-2AUa (Frogs, amphibians, animals)

    • Source

    • Rana aurora aurora (Northern red-legged frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • GILSSFKGVAKGVAKNLAGKLLDELKCKITGC

    • Sequence Length

    • 32

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.15325526]Gram-negative bacteria: Escherichia coli (MIC=5 µM), Pseudomonas aeruginosa (MIC=5 µM), Enterobacter cloacae (MIC=5 µM), Klebsiella pneumoniae (MIC=10 µM), P. mirabilis (MIC>40 µM);
      • Gram-positive bacteria: Staphylococcus aureus (MIC=20 µM), Staphylococcus epidermidis (MIC=20 µM), Enterococcus faecalis (MIC>40 µM), Streptococcus Group B (MIC=20 µM);
      • Yeast: Candida albicans (MIC>40 µM)

    • Hemolytic Activity

      • [Ref.15325526]HC50=290 μM against human erythrocytes

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02219 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C145H253N39O41S2

    • Absent Amino Acids

    • HMPQRWY

    • Common Amino Acids

    • K

    • Mass

    • 3262.96

    • PI

    • 9.51

    • Basic Residues

    • 6

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +4

    • Boman Index

    • -6.65

    • Hydrophobicity

    • 0.356

    • Aliphatic Index

    • 112.81

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 4.03

    • Polar Residues

    • 11

DRAMP02219

DRAMP02219 chydropathy plot

Comments Information

    • Function

    • Activity against a range of Gram-positive and Gram-negative bacteria (minimum inhibitory concentrations<20 µM) but low hemolytic activity against human erythrocytes.

Literature Information

  • ·Literature 1

    • Title

    • Host-defense peptides isolated from the skin secretions of the Northern red-legged frog Rana aurora aurora.

    • Reference

    • Dev Comp Immunol. 2005;29(1):83-90.

    • Author

    • Conlon JM, Sonnevend A, Davidson C, Demandt A, Jouenne T.