General Information

    • DRAMP ID

    • DRAMP02228

    • Peptide Name

    • RANATUERIN 1 (Frogs, amphibians, animals)

    • Source

    • Lithobates catesbeiana (American bullfrog) (Rana catesbeiana)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • SMLSVLKNLGKVGLGFVACKINKQC

    • Sequence Length

    • 25

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.9784389] Gram-positive bacterium: Staphylococcus aureus (MIC=50 µM);
      • Gram-negative bacterium: Escherichia coli (MIC=2 µM);
      • Yeast: Candida albicans (MIC=70 µM).

    • Hemolytic Activity

      • [Ref.9784389] It has no detectable hemolytic activity at aconcentration of 20µg/ml against human red blood cell.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys19 and Cys25)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys19 and Cys25.

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02228 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C117H204N32O31S3

    • Absent Amino Acids

    • DEHPRTWY

    • Common Amino Acids

    • KL

    • Mass

    • 2651.28

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +4

    • Boman Index

    • 1.42

    • Hydrophobicity

    • 0.596

    • Aliphatic Index

    • 116.8

    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.21

    • Polar Residues

    • 9

DRAMP02228

DRAMP02228 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacterium S.aureus and Gram-negative bacterium E.coli. Has activity against C.albicans. Has no detectable hemolytic activity.

Literature Information

  • ·Literature 1

    • Title

    • Ranatuerins: antimicrobial peptides isolated from the skin of the American bullfrog, Rana catesbeiana.

    • Reference

    • Biochem Biophys Res Commun. 1998 Sep 29;250(3):589-592.

    • Author

    • Goraya J, Knoop FC, Conlon JM.