General Information

    • DRAMP ID

    • DRAMP02244

    • Peptide Name

    • Ranatuerin-2Ca (Ranatuerin 2Ca; Frogs, amphibians, animals)

    • Source

    • Rana clamitans (Green frog) (Lithobates clamitans)

    • Family

    • Belongs to the frog skin active peptide (FSAP) family. Brevinin subfamily.

    • Gene

    • Not found

    • Sequence

    • GLFLDTLKGAAKDVAGKLLEGLKCKIAGCKP

    • Sequence Length

    • 31

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys24 and Cys29.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02244 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02244.

Physicochemical Information

    • Formula

    • C142H245N37O39S2

    • Absent Amino Acids

    • HMNQRSWY

    • Common Amino Acids

    • KL

    • Mass

    • 3158.85

    • PI

    • 9.19

    • Basic Residues

    • 6

    • Acidic Residues

    • 3

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +3

    • Boman Index

    • -4.16

    • Hydrophobicity

    • 0.268

    • Aliphatic Index

    • 110.32

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 4.17

    • Polar Residues

    • 8

DRAMP02244

DRAMP02244 chydropathy plot

Comments Information

    • Function

    • Antimicrobial peptide has defense response to Gram-positive bacterium, defense response to Gram-negative bacterium, male-specific defense response to bacterium, antibacterial humoral response, antibacterial humoral response, induced systemic resistance, antifungal innate immune response, antifungal humoral response, defense response to fungus (incompatible interaction) in GO analysis.

    • Tissue specificity

    • Expressed by the skin glands.

    • PTM

    • Disulfide bond at position 24 and 29.

Literature Information

  • ·Literature 1

    • Title

    • Purification and characterization of antimicrobial peptides from the skin of the North American green frog Rana clamitans.

    • Reference

    • Peptides. 2000 Apr;21(4):469-476.

    • Author

    • Halverson T, Basir YJ, Knoop FC, Conlon JM.