General Information
-
DRAMP ID
- DRAMP02290
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Peptide Name
- Gaegurin-RN5 (Frogs, amphibians, animals)
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Source
- Rana nigrovittata
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Family
- Not found
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Gene
- Not found
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Sequence
- FLGPIIKIATGILPTAICKFLKKC
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Sequence Length
- 24
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UniProt Entry
- No entry found
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Protein Existence
- Not found
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.19778602]Gram-positive bacteria: Staphylococcus aureus ATCC25923 (MIC=3.75 µg/ml), Staphylococcus aureus ATCC43300 (MIC=62.50 µg/ml), Bacillus subtilis (MIC=4.69 µg/ml);
- Gram-negative bacterium: Escherichia coli ML-35P (MIC=37.50 µg/ml);
- Yeast: Candida albicans ATCC2002 (MIC=4.69 µg/ml).
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Hemolytic Activity
-
- [Ref.19778602] It has no detectable hemolysis at the concentration up to 100 μg/ml
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
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N-terminal Modification
- Free
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C-terminal Modification
- Cyclization (Cys18 and Cys24)
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Nonterminal Modifications and Unusual Amino Acids
- Disulfide bond between Cys18 and Cys24.
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Stereochemistry
- L
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Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
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PDB ID
- None
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Predicted Structure
- Please click DRAMP02290_predicted_structure.pdb to download.
Physicochemical Information
-
Formula
- C124H210N28O27S2
Absent Amino Acids
- DEHMNQRSVWY
Common Amino Acids
- I
Mass
- 2589.32
PI
- 9.7
Basic Residues
- 4
Acidic Residues
- 0
Hydrophobic Residues
- 12
Net Charge
- +4
-
Boman Index
- 26.04
Hydrophobicity
- 1.129
Aliphatic Index
- 138.33
Half Life
-
- Mammalian:1.1 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 125
Absorbance 280nm
- 5.43
Polar Residues
- 6
DRAMP02290
Comments Information
Function
- Has hemolytic activity against rabbit red cells.
Literature Information
- ·Literature 1
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Title
- Peptidomics and genomics analysis of novel antimicrobial peptides from the frog, Rana nigrovittata.
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Pubmed ID
- 19778602
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Reference
- Genomics. 2010 Jan;95(1):66-71.
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Author
- Ma Y, Liu C, Liu X, Wu J, Yang H, Wang Y, Li J, Yu H, Lai R.