General Information

    • DRAMP ID

    • DRAMP02291

    • Peptide Name

    • Gaegurin-1 (Gaegurin 1; GGN1; Frogs, amphibians, animals)

    • Source

    • Glandirana rugosa (Japanese wrinkled frog) (Rana rugosa)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • GGN1

    • Sequence

    • SLFSLIKAGAKFLGKNLLKQGACYAACKASKQC

    • Sequence Length

    • 33

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.7999137]Gram-positive bacteria: Micrococcus luteus (MIC=5 µg/ml), Staphylococcus epidermidis (MIC=50 µg/ml), Bacillus subtilis (MIC=50 µg/ml);
      • Gram-negative bacteria: Klebsiella pneumoniae (MIC=100 µg/ml), Shigella dysentariae (MIC=25 µg/ml), Pseudomonas putida (MIC=50 µg/ml), Pseudomonas aeruginosa (MIC=50 µg/ml), Eshchericia coli (MIC=25 µg/ml).

    • Hemolytic Activity

      • [Ref.7999137] It has 0.04% hemolytic activity at 0.1 μg/ml, 0.14% hemolytic activity at 1 μg/ml, 0.51% hemolytic activity at 10 μg/ml, 0.59% hemolytic activity at 100 μg/ml against human red blood cells.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys27 and Cys33)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys27 and Cys33.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02291 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02291.

Physicochemical Information

    • Formula

    • C155H258N42O41S3

    • Absent Amino Acids

    • DEHMPRTVW

    • Common Amino Acids

    • AK

    • Mass

    • 3462.19

    • PI

    • 9.74

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 14

    • Net Charge

    • +6

    • Boman Index

    • -8.36

    • Hydrophobicity

    • 0.261

    • Aliphatic Index

    • 89.09

    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1615

    • Absorbance 280nm

    • 50.47

    • Polar Residues

    • 11

DRAMP02291

DRAMP02291 chydropathy plot

Comments Information

    • Function

    • Has hemolytic activity against rabbit red cells.

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial peptides from the skin of a Korean frog, Rana rugosa.

    • Reference

    • Biochem Biophys Res Commun. 1994 Nov 30;205(1):948-954.

    • Author

    • Park JM, Jung J-E, Lee BJ.1994