General Information

    • DRAMP ID

    • DRAMP02295

    • Peptide Name

    • Gaegurin-5 (Gaegurin 5; GGN5; Brevinin-1EMa; Frogs, amphibians, animals)

    • Source

    • Glandirana rugosa (Japanese wrinkled frog) (Rana rugosa)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • GGN5

    • Sequence

    • FLGALFKVASKVLPSVFCAITKKC

    • Sequence Length

    • 24

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antiprotozoal

    • Target Organism

      • [Ref.7999137]Gram-positive bacteria: Micrococcus luteus (MIC=2.5 µg/ml), Staphylococcus epidermidis (MIC=10 µg/ml), Bacillus subtilis (MIC=10 µg/ml);
      • Gram-negative bacteria: Klebsiella pneumoniae (MIC=50 µg/ml), Shigella dysentariae (MIC=50 µg/ml), Pseudomonas putida (MIC=50 µg/ml), Pseudomonas aeruginosa (MIC=100 µg/ml), Eshchericia coli (MIC=50 µg/ml);
      • Fungi: Saccharomyces cerevisiae (MIC=50 µg/ml), Candida albicans (MIC=50 µg/ml), Salmonella typhimurium (MIC=200 µg/ml), Proteus mirabilis (MIC>200 µg/ml).

    • Hemolytic Activity

      • [Ref.7999137] It has 0.19% hemolytic activity at 0.1 μg/ml, 0.31% hemolytic activity at 1 μg/ml, 0.78% hemolytic activity at 10 μg/ml, 1.01% hemolytic activity at 100 μg/ml against human red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys18 and Cys24)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys18 and Cys24.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02295 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02295.

Physicochemical Information

    • Formula

    • C122H200N28O28S2

    • Absent Amino Acids

    • DEHMNQRWY

    • Common Amino Acids

    • K

    • Mass

    • 2571.22

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +4

    • Boman Index

    • 18.1

    • Hydrophobicity

    • 1.142

    • Aliphatic Index

    • 113.75

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.43

    • Polar Residues

    • 6

DRAMP02295

DRAMP02295 chydropathy plot

Comments Information

    • Function

    • Has low hemolytic activity. Has a broad spectrum of activity against both Gram-positive and Gram-negative bacteria, fungi and protozoa. PTM

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial peptides from the skin of a Korean frog, Rana rugosa.

    • Reference

    • Biochem Biophys Res Commun. 1994 Nov 30;205(1):948-954.

    • Author

    • Park JM, Jung JE, Lee BJ.