• • DRAMP ID

  • DRAMP02296

• • Peptide Name

  • Gaegurin-6 (Gaegurin 6; GGN6; Frogs, amphibians, animals)

• • Source

  • Glandirana rugosa (Japanese wrinkled frog) (Rana rugosa)

• • Family

  • Belongs to the frog skin active peptide family (Brevinin subfamily)

• • Gene

  • GGN6

• • Sequence

  • FLPLLAGLAANFLPTIICKISYKC

• • Sequence Length

  • 24

• • UniProt Entry

  • P80400

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.7999137]Gram-positive bacteria: Micrococcus luteus (MIC=2.5 µg/ml), Staphylococcus epidermidis (MIC=10 µg/ml), Bacillus subtilis (MIC=10 µg/ml);
    • Gram-negative bacteria: Klebsiella pneumoniae (MIC=50 µg/ml), Shigella dysentariae (MIC=50 µg/ml), Pseudomonas putida (MIC=50 µg/ml), Pseudomonas aeruginosa (MIC=100 µg/ml), Eshchericia coli (MIC=50 µg/ml);
    • Fungi: Saccharomyces cerevisiae (MIC=50 µg/ml), Candida albicans (MIC=50 µg/ml), Salmonella typhimurium (MIC=200 µg/ml), Proteus mirabilis (MIC>200 µg/ml), Serratia marcescens (MIC>200 µg/ml).

• • Hemolytic Activity

  • • [Ref.7999137] It has 0.39% hemolytic activity at 0.1 μg/ml, 0.62% hemolytic activity at 1 μg/ml, 0.78% hemolytic activity at 10 μg/ml, 0.86% hemolytic activity at 100 μg/ml against human red blood cells

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Cyclization (Cys18 and Cys24)

• • Nonterminal Modifications and Unusual Amino Acids

  • Disulfide bond between Cys18 and Cys24.

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP02296.

DRAMP02296

• • Function

  • Has low hemolytic activity. Has a broad spectrum of activity against both Gram-positive and Gram-negative bacteria, fungi and protozoa. PTM

• ·Literature 1

• • Title

  • Antimicrobial peptides from the skin of a Korean frog, Rana rugosa.

• • Pubmed ID

  • 7999137

• • Reference

  • Biochem Biophys Res Commun. 1994 Nov 30;205(1):948-954.

• • Author

  • Park JM, Jung JE, Lee BJ.