General Information

    • DRAMP ID

    • DRAMP02321

    • Peptide Name

    • Grammistin Pp3 (Group III grammistin; fish, chordates, animals)

    • Source

    • Pogonoperca punctata (Clown grouper) (Bearded grouper)

    • Family

    • Belongs to the grammistin family (Group 3 subfamily)

    • Gene

    • Not found

    • Sequence

    • NWRKILGQIASVGAGLLGSLLAGYE

    • Sequence Length

    • 25

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.Fish. Sci. 2001 Oct;67:928-933.]Gram-positive bacteria: Bacillus subtilis IAM 1026 (MIC=50 µg/ml), Staphylococcus aureus IAM 1011 (MIC=50 µg/ml);
      • Gram-negative bacteria: Aeromonas salmonicida NCIBM 1102 (MIC=50 µg/ml), Escherichia coli JCM 1649 (MIC>50 µg/ml), P. dameselae subsp. Piscicida Sp 9587 (MIC=25 µg/ml), Salmonella typhimurium S1 (MIC>50 µg/ml), S. putrefaciens IAM 1509 (MIC=12.5 µg/ml), Vibrio anguillarum NCIBM 829 (MIC=50 µg/ml), Vibrio parahaemolyticus (MIC=50 µg/ml).

    • Hemolytic Activity

      • [Ref.Fish. Sci. 2001 Oct;67:928-933.]75 HU/mg hemolytic activity against horse erythrocytes(1 HU was defined as the amount of peptide causing 50% hemolysis)

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02321 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02321.

Physicochemical Information

    • Formula

    • C118H192N32O33

    • Absent Amino Acids

    • CDFHMPT

    • Common Amino Acids

    • GL

    • Mass

    • 2587.02

    • PI

    • 8.59

    • Basic Residues

    • 2

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 12

    • Net Charge

    • +1

    • Boman Index

    • 4.54

    • Hydrophobicity

    • 0.516

    • Aliphatic Index

    • 132.8

    • Half Life

      • Mammalian:1.4 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 6990

    • Absorbance 280nm

    • 291.25

    • Polar Residues

    • 9

DRAMP02321

DRAMP02321 chydropathy plot

Comments Information

    • Function

    • Has hemolytic activity. Thanks to its abundant amphiphilic alpha-helices, it may integrate into membrane phospholipids, leading to lysis of the membrane.

Literature Information

  • ·Literature 1

    • Title

    • Interaction of grammistins with lipids and their antibacterial activity.

    • Reference

    • Fish. Sci. 2001 Oct;67:928-933.

    • Author

    • Yokota H, Nagashima Y, Shiomi K.