General Information

    • DRAMP ID

    • DRAMP02445

    • Peptide Name

    • Antimicrobial protein BL-A60

    • Source

    • Brevibacillus laterosporus (Bacillus laterosporus)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • LYKLVKVVLNM

    • Sequence Length

    • 11

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-negative bacterium: Pseudomonas solanacearum (MIC=10 µM);
      • Gram-positive bacterium: Bacillus subtilis (MIC=5 µM).
      • Fungi: Phytophthora capsici (MIC=15 µM), Botrytis cinerea (MIC=25 µM), Verticillium dahliae (MIC=200 µM), Fusarium oxysporum (MIC=50 µM), Xerocomus vesicolor (MIC=100 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02445 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02445.

Physicochemical Information

    • Formula

    • C63H110N14O14S

    • Absent Amino Acids

    • ACDEFGHIPQRSTW

    • Common Amino Acids

    • LV

    • Mass

    • 1319.71

    • PI

    • 9.7

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +2

    • Boman Index

    • 11.35

    • Hydrophobicity

    • 1.209

    • Aliphatic Index

    • 185.45

    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 149

    • Polar Residues

    • 2

DRAMP02445

Comments Information

    • Function

    • Has antibacterial and antifungal activity. In P.capsici, inhibits mycelial growth (EC50=7.89 µg/ml), formation of sporangia (EC50=0.6 µg/ml) and cytospore germination (EC50=21.96 µg/ml).

    • PTM

    • Contains a choline moiety at the N-terminus and a tenuazonic acid moiety at the C-terminus.

    • Biophysicochemical properties

    • pH dependence (Active between pH 3 and 11); Temperature dependence (Retains >80% activity after 15 min at 100 degrees Celsius).

Literature Information

  • ·Literature 1

    • Title

    • Purification and characterization of a novel antimicrobial peptide from Brevibacillus laterosporus strain A60.

    • Reference

    • Peptides. 2012 Feb;33(2):206-211.

    • Author

    • Zhao J, Guo L, Zeng H, Yang X, Yuan J, Shi H, Xiong Y, Chen M, Han L, Qiu D.