General Information

    • DRAMP ID

    • DRAMP02779

    • Peptide Name

    • Defensin-A (Defensin A; Insects, animals)

    • Source

    • Anomala cuprea (Cupreous chafer beetle)

    • Family

    • Belongs to the invertebrate defensin family (Type 1 subfamily)

    • Gene

    • Not found

    • Sequence

    • VTCDLLSFEAKGFAANHSICAAHCLAIGRKGGSCQNGVCVCRN

    • Sequence Length

    • 43

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacteria: Bacillus megaterium (MIC=1.2-2.5 µM), Bacillus subtilis (MIC>40 µM), Micrococcus luteus (MIC=1.2-2.5 µM), Staphylococcus aureus (MIC=2.5-5 µM).
      • Gram-negative bacteria: Enterobacter asbriae (MIC>40 µM), Escherichia coli (MIC>40 µM), Pseudomonas aeruginosa (MIC>40 µM), Serratia marcescens (MIC>40 µM), Xenorhabdus japonicus (MIC>40 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys3 and Cys34; Cys20 and Cys39; Cys24 and Cys41.

    • Stereochemistry

    • L

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02779 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02779.

Physicochemical Information

    • Formula

    • C184H299N59O56S6

    • Absent Amino Acids

    • MPWY

    • Common Amino Acids

    • AC

    • Mass

    • 4426.12

    • PI

    • 8.34

    • Basic Residues

    • 6

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 16

    • Net Charge

    • +4

    • Boman Index

    • -38.1

    • Hydrophobicity

    • 0.351

    • Aliphatic Index

    • 79.53

    • Half Life

      • Mammalian:100 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 375

    • Absorbance 280nm

    • 8.93

    • Polar Residues

    • 18

DRAMP02779

DRAMP02779 chydropathy plot

Comments Information

    • Function

    • Strong activity against the Gram-positive bacteria M.luteus, B.megaterium and S. aureus. Reduced activity against Gram-positive bacterium B.subtilis and weak activity against Gram-negative bacterium X.japonicus.

    • PTM

    • Problely contains three disulfide bonds 3-34; 20-39; 24-41.

Literature Information

  • ·Literature 1

    • Title

    • Two novel insect defensins from larvae of the cupreous chafer, Anomala cuprea: purification, amino acid sequences and antibacterial activity.

    • Reference

    • Insect Biochem Mol Biol. 2001 Dec;32(1):75-84.

    • Author

    • Yamauchi H.