General Information

    • DRAMP ID

    • DRAMP02855

    • Peptide Name

    • Cathelicidin-6 (Antibacterial peptide BMAP-27, Myeloid antib

    • Source

    • Bos taurus (Bovine)

    • Family

    • Belongs to the cathelicidin family

    • Gene

    • CATHL6

    • Sequence

    • GRFKRFRKKFKKLFKKLSPVIPLLHLG

    • Sequence Length

    • 27

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-negative bacteria: Escherichia coli ATCC25922 (MIC=1 µg/ml), E. coli ML35 (MIC=1 µg/ml), E. coli D21 (MIC=0.25 µg/ml), Salmonella typhimurium ATCC 14028 (MIC=1 µg/ml), Pseudomonas aeruginosa ATCC 27853 (MIC=1 µg/ml), Serratia marcescens ATCC 8100 (MIC=2 µg/ml);
      • Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (MIC=2 µg/ml), Staphylococcus aureus Cowan 1 (MIC=2 µg/ml), Staphylococcus aureus (MRSA) (MIC=4 µg/ml), S. epidermidis ATCC 12228 (MIC=1 µg/ml), Bacillus megaterium Bm11 (MIC=2 µg/ml).
      • Fungi: Candida albicans (MIC=8 µg/ml), Cryptococcus neoformans (MIC=4 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix (2 helices; 19 residues)

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02855 helical wheel diagram

    • PDB ID

    • 2KET resolved by NMR.
  • 2KET-> 

    • Predicted Structure

Physicochemical Information

    • Formula

    • C160H265N45O29

    • Absent Amino Acids

    • ACDEMNQTWY

    • Common Amino Acids

    • K

    • Mass

    • 3283.15

    • PI

    • 12.32

    • Basic Residues

    • 11

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +11

    • Boman Index

    • -44.31

    • Hydrophobicity

    • -0.367

    • Aliphatic Index

    • 97.41

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP02855

DRAMP02855 chydropathy plot

Comments Information

    • Function

    • Exerts a potent antimicrobial activity against Gram-negative and Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus, and fungi.

    • PTM

    • Contains two disulfide bonds. (By similarity)

Literature Information

  • ·Literature 1

    • Title

    • Biological characterization of two novel cathelicidin-derived peptides and identification of structural requirements for their antimicrobial and cell lytic activities.

    • Reference

    • J. Biol. Chem. 1996;271:28375-28381.

    • Author

    • Skerlavaj B, Gennaro R, Bagella L, Merluzzi L, Risso A, Zanetti M.
  • ·Literature 2

    • Title

    • Sequence analysis and polymorphism discovery in 4 members of the bovine cathelicidin gene family.

    • Reference

    • J. Hered. 2009;100:241-245.

    • Author

    • Gillenwaters EN, Seabury CM, Elliott JS, Womack JE.
  • ·Literature 3

    • Title

    • solution structure of BMAP-27.

    • Reference

    • To be Published

    • Author

    • Yang S, Jung H, Kim J.