General Information
-
DRAMP ID
- DRAMP02860
-
Peptide Name
- Bovine Beta-defensin 3 (bBD-3; BNBD-3; BNDB-3; mammals, animals)
-
Source
- Bos taurus (Bovine)
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Family
- Belongs to the beta-defensin family
-
Gene
- DEFB3
-
Sequence
- EGVRNHVTCRINRGFCVPIRCPGRTRQIGTCFGPRIKCCRSW
-
Sequence Length
- 42
-
UniProt Entry
- P46161
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- Gram-positive bacterium: Staphylococcus aureus 502A (MIC=10-300 µg/ml);
- Gram-negative bacterium: Escherichia coli ML35 (MIC=10-300 µg/ml).
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
-
N-terminal Modification
- Not included yet
-
C-terminal Modification
- Not included yet
-
Nonterminal Modifications and Unusual Amino Acids
- Not included yet
-
Stereochemistry
- Not included yet
-
Structure
- Bridge
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP02860.
Physicochemical Information
-
Formula
- C204H335N73O52S6
Absent Amino Acids
- ADLMY
Common Amino Acids
- R
Mass
- 4834.72
PI
- 10.63
Basic Residues
- 10
Acidic Residues
- 1
Hydrophobic Residues
- 10
Net Charge
- +9
-
Boman Index
- -113.84
Hydrophobicity
- -0.393
Aliphatic Index
- 57.86
Half Life
-
- Mammalian:1 hour
- Yeast:30 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 5875
Absorbance 280nm
- 143.29
Polar Residues
- 17
DRAMP02860
Comments Information
Function
- Has bactericidal activity. Active against E. coli ML35 and S. aureus 502A.
Tissue specificity
- Neutrophilic granules.
PTM
- Contains three disulfide bonds 9-38; 16-31; 21-39. (By similarity)
Literature Information
- ·Literature 1
-
Title
- Purification, primary structures, and antibacterial activities of beta-defensins, a new family of antimicrobial peptides from bovine neutrophils.
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Pubmed ID
- 8454635
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Reference
- J Biol Chem. 1993 Mar 25;268(9):6641-6648.
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Author
- Selsted ME, Tang YQ, Morris WL, McGuire PA, Novotny MJ, Smith W, Henschen AH, Cullor JS.
- ·Literature 2
-
Title
- Enteric beta-defensin: molecular cloning and characterization of a gene with inducible intestinal epithelial cell expression associated with Cryptosporidium parvum infection.
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Pubmed ID
- 9488394
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Reference
- Infect Immun. 1998 Mar;66(3):1045-1056.
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Author
- Tarver AP, Clark DP, Diamond G, Russell JP, Erdjument-Bromage H, Tempst P, Cohen KS, Jones DE, Sweeney RW, Wines M, Hwang S, Bevins CL.