General Information

    • DRAMP ID

    • DRAMP02865

    • Peptide Name

    • Bovine Beta-defensin 8 (bBD-8; BNBD-8; BNDB-8; mammals, animals)

    • Source

    • Bos taurus (Bovine)

    • Family

    • Belongs to the beta-defensin family

    • Gene

    • DEFB8

    • Sequence

    • VRNFVTCRINRGFCVPIRCPGHRRQIGTCLGPQIKCCR

    • Sequence Length

    • 38

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacterium:
        Target OrganismActivity
        Staphylococcus aureus 502A (MIC=10-300 µg/ml);-
      • Gram-negative bacterium:
        Target OrganismActivity
        Escherichia coli ML35MIC=10-300 µg/ml

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02865 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02865.

Physicochemical Information

    • Formula

    • C184H310N66O45S6

    • Absent Amino Acids

    • ADEMSWY

    • Common Amino Acids

    • R

    • Mass

    • 4359.26

    • PI

    • 10.66

    • Basic Residues

    • 9

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +9

    • Boman Index

    • -90.03

    • Hydrophobicity

    • -0.142

    • Aliphatic Index

    • 74.21

    • Half Life

      • Mammalian:100 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 375

    • Absorbance 280nm

    • 10.14

    • Polar Residues

    • 14

DRAMP02865

DRAMP02865 chydropathy plot

Comments Information

    • Function

    • Has bactericidal activity. Active against E. coli ML35 and S. aureus 502A.

    • Tissue specificity

    • Neutrophilic granules.

    • PTM

    • Contains three disulfide bonds 7-36; 14-29; 19-37. (By similarity)

Literature Information

  • ·Literature 1

    • Title

    • Purification, primary structures, and antibacterial activities of Beta-defensins, a new family of antimicrobial peptides from bovine neutrophils.

    • Reference

    • J Biol Chem. 1993 Mar 25;268(9):6641-6648.

    • Author

    • Selsted ME, Tang YQ, Morris WL, McGuire PA, Novotny MJ, Smith W, Henschen AH, Cullor JS.