General Information

    • DRAMP ID

    • DRAMP02960

    • Peptide Name

    • Antibacterial peptide PMAP-23 (Myeloid antibacterial peptide 23; pigs, mammals, animals)

    • Source

    • Sus scrofa (Pig)

    • Family

    • Belongs to the cathelicidin family

    • Gene

    • PMAP23

    • Sequence

    • RIIDLLWRVRRPQKPKFVTVWVR

    • Sequence Length

    • 23

    • Protein Existence

    • Transcript level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-negative bacteria:
        Target OrganismActivity
        Escherichia coli ML35MIC=2 µM
        Escherichia coli ATCC 25922MIC=4 µM
        Salmonella typhimurium ATCC 14028MIC=8 µM
        Pseudomonas aeruginosa ATCC 27853MIC=16 µM
      • Gram-positive bacteria:
        Target OrganismActivity
        Bacillus megateriumMIC=2 µM
        Staphylococcus aureus ATCC 25923MIC=4 µM

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02960 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02960.

Physicochemical Information

    • Formula

    • C140H229N43O28

    • Absent Amino Acids

    • ACEGHMNSY

    • Common Amino Acids

    • R

    • Mass

    • 2962.63

    • PI

    • 12.18

    • Basic Residues

    • 7

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +6

    • Boman Index

    • -59.05

    • Hydrophobicity

    • -0.296

    • Aliphatic Index

    • 118.26

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 11000

    • Absorbance 280nm

    • 500

    • Polar Residues

    • 1

DRAMP02960

DRAMP02960 chydropathy plot

Comments Information

    • Function

    • Exerts antimicrobial activity against both Gram-positive and negative bacteria at concentrations of 2-16 micro molar. Its activity appears to be mediated by its ability to damage bacterial membranes.

    • PTM

    • Contains two disulfide bonds (By similarity).

Literature Information

  • ·Literature 1

    • Title

    • Molecular cloning and chemical synthesis of a novel antibacterial peptide derived from pig myeloid cells.

    • Reference

    • J Biol Chem. 1994 Mar 18;269(11):7855-7858.

    • Author

    • Zanetti M, Storici P, Tossi A, Scocchi M, Gennaro R.