General Information

    • DRAMP ID

    • DRAMP03750

    • Peptide Name

    • Venom antimicrobial peptide-6 (Meucin-13; NDBP-5; Arthropods, animals)

    • Source

    • Mesobuthus eupeus (Lesser Asian scorpion)

    • Family

    • Belongs to the scorpion NDBP 5 family

    • Gene

    • Not found

    • Sequence

    • IFGAIAGLLKNIF

    • Sequence Length

    • 13

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.19088182]Gram-positive bacteria: Bacillus sp. DM-1 (MIC=2 µM), Micrococcus luteus (MIC=2.9 µM), Bacillus megaterium (MIC=0.25 µM);
      • Gram-negative bacteria: Agrobacterium tumefaciens (MIC=11.8 µM), Escherichia coli (MIC=7.90 µM), Shewanella oneidensis (MIC=6.20 µM), Stenotrophomonus sp. YC-1 (MIC=6.20 µM);
      • Fungi: Beauveria spp. (MIC=14.1 µM), Neurospora crassa (MIC=18.3 µM), Candida albicans (MIC=42.8 µM), Saccharomyces cerevisiae (MIC=18.3 µM).

    • Hemolytic Activity

      • [Ref.19088182]37.7% hemolytic activity at 6.25 M, 59.2% hemolytic activity at 12.5 M against rabbit red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03750 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03750.

Physicochemical Information

    • Formula

    • C68H109N15O15

    • Absent Amino Acids

    • CDEHMPQRSTVWY

    • Common Amino Acids

    • I

    • Mass

    • 1376.7

    • PI

    • 8.75

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +1

    • Boman Index

    • 23.87

    • Hydrophobicity

    • 1.7

    • Aliphatic Index

    • 165.38

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP03750

Comments Information

    • Function

    • Amphipathic peptide that exhibits extensive cytolytic activities against both prokaryotic and eukaryotic cells. Is more potent against Gram-positive bacteria (lethal concentration (LC=0.25-2.9 µM) than against Gram-negative bacteria (LC=6.2->50 µM), and fungi (LC=14.1->50 µM). Shows hemolytic activity against rabbit erythrocytes and cytolysis against rat dorsal root ganglions. In vivo, intravenous injection into mice tail provokes uncomfortable symptoms with a death rate of 12.5%.

    • Tissue specificity

    • Expressed by the venom gland.

Literature Information

  • ·Literature 1

    • Title

    • Structural and functional characterization of two genetically related meucin peptides highlights evolutionary divergence and convergence in antimicrobial peptides.

    • Reference

    • FASEB J. 2009 Apr;23(4):1230-1245.

    • Author

    • Gao B, Sherman P, Luo L, Bowie J, Zhu S.