Patent Information

    • DRAMP ID

    • DRAMP08913

    • Sequence

    • MCHWAGGASNTGDARGDVFGKQAG

    • Sequence Length

    • 24

    • Sequence Name

    • Sequence 5 from Patent US 20090048160

    • Source

    • Bos sp.

    • Activity

    • Antimicrobial, Antibacterial

    • Patent Type

    • Patent Application

    • Publication Date

    • 2009-2-19

    • Patent Title

    • Antimicrobial activity of bovine bactericidal/permeability-increasing protein (BPI)-derived peptides against Gram-negative bacterial mastitis

    • Abstract

    • The antimicrobial activity of bovine bactericidal/permeability-increasing protein (bBPI)-derived synthetic peptides against mastitis-causing Gram-negative bacteria was evaluated. Three peptides were synthesized with sequences corresponding to amino acids 65-99 (bBPI65-99), 142-169 (bBPI142-169), or the combination of amino acids 90-99 and 148-161 (bBPI90-99,148-161) of bBPI. The bBPI90-99,148-161 peptide demonstrated the widest spectrum of antimicrobial activity, with minimum inhibitory (MIC) and bactericidal (MBC) concentration values ranging from 16-64 µg/ml against Escherichia coli, Klebsiella pneumoniae, and Enterobacter spp, and 64-128 µg/ml against Pseudomonas aeruginosa. None of the peptides exhibited any growth inhibitory effect on Serratia marcescens. The antimicrobial activity of bBPI90-99,148-161 was inhibited in milk, but preserved in serum. Finally, both bBPI142-169 and bBPI90-99,148-161 were demonstrated to completely neutralize LPS. The peptide bBPI90-99,148-161 is a potent neutrali