General Information

    • DRAMP ID

    • DRAMP18128

    • Peptide Name

    • Antimicrobial peptide HsAp4;

    • Source

    • Heterometrus spinifer (Asia giant forest scorpion) (Malaysian blackscorpion)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • SGTSEKERESGRLLGVVKRLIVGFRSPFR

    • Sequence Length

    • 29

    • Protein Existence

    • Transcript level

Activity Information

    • Biological Activity

    • Antimicrobial, Antifungal, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.23000095]Gram-positive bacteria: Staphylococcus aureus AB 94004 (MIC=23.6µM), Bacillus magaterium AB 90008 (MIC=11.8µM), Bacillus thuringiensis AB 93100 (MIC=46.5µM);
      • Gram-negative bacteria: Salmonella enterica AB 2010185 (MIC=25.0µM), E. coli DH5 (MIC=51.2µM), E. coli JM109 (MIC=50.4µM), Enterobacter cloacae subsp. cloacae AB 2010162 (MIC=47.9µM), Klebsiella oxytoca AB 2010143 (MIC=50.5µM);
      • Fungi: Candida tropicalis AY 91009 (MIC=48.6µM).

    • Hemolytic Activity

      • [Ref.23000095]5% hemolytic activity at 0.8 μM, 35% hemolytic activity at 1.6 μM, 95% hemolytic activity at 3.2 μM, 100% hemolytic activity against human red blood cells.

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP18128 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C375H643N115O104S4

    • Absent Amino Acids

    • CDHN

    • Common Amino Acids

    • R

    • Mass

    • 3261.78

    • PI

    • 11.44

    • Basic Residues

    • 17

    • Acidic Residues

    • 7

    • Hydrophobic Residues

    • 24

    • Net Charge

    • +10

    • Boman Index

    • -10000

    • Hydrophobicity

    • -0.292

    • Aliphatic Index

    • 93.38

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 6990

    • Absorbance 280nm

    • 95.75

    • Polar Residues

    • 20

DRAMP18128

DRAMP18128 chydropathy plot

Comments Information

    • Function

    • Possesses antimicrobial activity against both Gram- negative and Gram-positive bacteria, as well as against the fungus C. tropicaliS. Also possess a relatively high hemolytic activity (By similarity).

Literature Information

  • ·Literature 1

    • Title

    • A novel class of antimicrobial peptides from the scorpionHeterometrus spinifer.

    • Reference

    • Peptides 38:389-394 (2012).

    • Author

    • Nie Y., Zeng X.C. , Yang Y., Luo F., Luo X., Wu S. , Zhang L., Zhou J.