General Information

    • DRAMP ID

    • DRAMP18323

    • Peptide Name

    • Nukacin ISK-1(Bacteriocin)

    • Source

    • Staphylococcus warneri ISK-1

    • Family

    • Belongs to the lantibiotics family (Class I bacteriocin)

    • Gene

    • nukA

    • Sequence

    • KKKSGVIPTVSHDCHMNSFQFVFTCCS

    • Sequence Length

    • 27

    • Protein Existence

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

    • Gram-positive

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Lipid II

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • There are one didehydrobutyrine (Dhb): T24; two lanthionines: S11-C25, S18-C26; and one Beta-methyllanthionine: T9-C14.

    • Helical Wheel Diagram

    • DRAMP18323 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP18323.

Physicochemical Information

    • Formula

    • C132H204N36O38S4

    • Absent Amino Acids

    • AELRWY

    • Common Amino Acids

    • S

    • Mass

    • 3031.53

    • PI

    • 8.66

    • Basic Residues

    • 5

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +4

    • Boman Index

    • -3250

    • Hydrophobicity

    • -0.011

    • Aliphatic Index

    • 46.67

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 4.81

    • Polar Residues

    • 11

DRAMP18323

DRAMP18323 chydropathy plot

Comments Information

    • Nukacin 3299, produced by Staphylococcus simulans 3299, is identical to nukacin ISK-1.Partial known sequence of Warnericin RB4 matches nukacin ISK-1, suggesting a possible identical peptide also produced by Staphylococcus warneri RB4.

Literature Information

  • ·Literature 1

    • Title

    • A novel lantibiotic, nukacin ISK-1, of Staphylococcus warneri ISK-1: cloning of the structural gene and identification of the structure.

    • Reference

    • Biosci Biotechnol Biochem. 2000 Nov;64(11):2420-8.

    • Author

    • Sashihara T, Kimura H, Higuchi T, Adachi A, Matsusaki H, Sonomoto K, Ishizaki A.
  • ·Literature 2

    • Title

    • Nukacin 3299, a lantibiotic produced by Staphylococcus simulans 3299 identical to nukacin ISK-1.

    • Reference

    • Vet Microbiol. 2010 Nov 20;146(1-2):124-31.

    • Author

    • Ceotto H, Holo H, da Costa KF, Nascimento Jdos S, Salehian Z, Nes IF, Bastos Mdo C.