General Information

    • DRAMP ID

    • DRAMP18665

    • Peptide Name

    • Mastoparan-L (MP-L; insects, arthropods, invertebrates, animals)

    • Source

    • Vespula lewisii (Korean yellow-jacket isp) (Vespula flaviceps lewisii)

    • Family

    • Belongs to the tetradecapeptide family

    • Gene

    • Not found

    • Sequence

    • INLKALAALAKKIL

    • Sequence Length

    • 14

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.27423268]Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (MIC=12.5 μM), Enterococcus faecalis ATCC 29212 (MIC=25 μM);
      • Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=25 μM), Pseudomonas aeruginosa ATCC 27853 (MIC=50 μM), Klebsiella pneumoniae ATCC 13883 (MIC=12.5 μM), Acinetobacter baumannii ATCC 19606 (MIC=2.7 μM)

    • Hemolytic Activity

      • [Ref.27423268]40% hemolysis at 10μM against rat red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP18665 helical wheel diagram

    • PDB ID

    • 1D7N resolved by NMR.
  • 1D7N-> 

    • Predicted Structure

Physicochemical Information

    • Formula

    • C70H130N18O16

    • Absent Amino Acids

    • CDEFGHMPQRSTVWY

    • Common Amino Acids

    • AL

    • Mass

    • 1479.91

    • PI

    • 10.3

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +3

    • Boman Index

    • 1347

    • Hydrophobicity

    • 1.157

    • Aliphatic Index

    • 195.71

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 1

DRAMP18665

Comments Information

    • Function

    • Mast cell degranulating peptide. Activates G proteins that couple to phospholipase C. May be able to switch from an in-plane to a transmembrane orientation in lipid bilayers. Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Has hemolytic activity.

    • PTM

    • Leucine amide at the position 14.

    • Tissue specificity

    • Expressed by the venom gland.

Literature Information

  • ·Literature 1

    • Title

    • Selective amino acid substitution reduces cytotoxicity of the antimicrobial peptide mastoparan.

    • Reference

    • Biochim Biophys Acta. 2016 Nov;1858(11):2699-2708.

    • Author

    • Irazazabal LN, Porto WF, Ribeiro SM, Casale S, Humblot V, Ladram A, Franco OL.