General Information

    • DRAMP ID

    • DRAMP20800

    • Peptide Name

    • the K4 peptide (synthetic; Phe-rich >25%)

    • Source

    • the APD database-aided design.

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • KKKKPLFGLFFGLF

    • Sequence Length

    • 14

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

    • SMILES

    • CC(C)C[C@H](NC(=O)CNC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)O

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Target Organism

      • [Ref.19573572] Gram-negative bacteria:
        Target OrganismActivity
        Escherichia coliMIC=40-80 μg/ml or 24-48 μM
        Vibrio alginolyticusMIC=10-20 μg/ml or 6-12 μM
        Vibrio harveyiMIC=5-10 μg/ml or 3-6 μM

    • Hemolytic Activity

      • [Ref.19573572] No significant hemolytic activity is observed at 10 and 40 μg/ml. Slight hemolysis (6.65%) is shown at 160 μg/ml.

    • Cytotoxicity

      • [Ref.19573572] The cell viability of CHO-K1 cells is 127%, 123%,78% and 10% at peptide concentrations of 10 μg/ml, 40 μg/ml, 160 μg/ml and 640 μg/ml

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • PDB ID

    • Predicted Structure

    • There is no predicted structure for DRAMP20800.

Physicochemical Information

    • Formula

    • C87H132N18O15

    • Absent Amino Acids

    • ACDEHIMNQRSTVWY

    • Common Amino Acids

    • FK

    • Mass

    • 1670.12

    • PI

    • 10.48

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +4

    • Boman Index

    • 636

    • Hydrophobicity

    • 0.329

    • Aliphatic Index

    • 83.57

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP20800

Comments Information

    • Function

    • Antibacterial activity against the Gram-positive and Gram-negative bacteria. Have Hemolytic activity against rabbit erythrocytes. Have Cytotoxicity activity on CHO-K1 cells, the peptide is slightly cytotoxic at 160

Literature Information

  • ·Literature 1

    • Title

    • KKKKPLFGLFFGLF: a cationic peptide designed to exert antibacterial activity

    • Reference

    • Peptides. 2009 Sep;30(9):1608-12

    • Author

    • Duval E, Zatylny C, Laurencin M, Baudy-Floc'h M, Henry J.