General Information
-
DRAMP ID
- DRAMP21526
-
Peptide Name
- Mag(i+4)15(F5K)
-
Sequence
- GIGKKLHSAKKFGKAⓍVGEⓍJNS
-
Sequence Length
- 23
-
Original Sequence
- GIGKFLHSAKKFGKAFVGEIMNS
-
Source
- Synthetic construct
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram-
-
Comments
- Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 50 μg/mL.
-
Target Organism
-
- [Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC > 50 μg/mL);
- Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.69 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 17.68 μg/mL)
-
Hemolytic Activity
-
- [Ref.31427820] It has 1.5% hemolysis against human red blood cells at 25 μg/mL.
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
Structure Information
-
Linear/Cyclic
- Cyclic (Stapled)
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Special Amino Acid and Stapling Position
- ①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.
-
Stereochemistry
- L
-
Secondary Structure
- α-helix in potassium phosphate buffer.
-
Structure Description
- No other descriptive information about the structure found in the literature
-
Helical Wheel Diagram
-
Predicted Structure
- There is no predicted structure for DRAMP21526.
Physicochemical Information
-
Formula
- C₁₁₁H₁₈₇N₃₁O₂₉
Absent Amino Acids
- CDMPQRTWY
Common Amino Acids
- K
Mass
- 2419.9
PI
- /
-
Basic Residues
- 6
Acidic Residues
- 1
Hydrophobic Residues
- 6
Hydrophobicity
- /
Polar Residues
- 7
DRAMP21526
Literature Information
- Literature 1
-
Title
- Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice
-
Pubmed ID
- 31427820
-
Reference
- Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.
-
Author
- Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky