• • DRAMP ID

  • DRAMP30318

• • Peptide Name

  • Temporin L[Pro3,DLeu9,DHyp10](TL5)

• • Source

  • Synthetic construct(derived from Temporin-L)

• • Family

  • Coronaviridae, Herpesviridae, Paramyxoviridae

• • Gene

  • Not found

• • Sequence

  • FVPWFSKFlxRIL

• • Sequence Length

  • 13

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Not found

• • Biological Activity

  • Antimicrobial, Antiviral

• • Target Organism

  • • [Ref.35216177]HSV-1:

      Target Organism	Activity
      inhibition of HSV-1 replication in Vero cells(IC50>50.00 μM,IC90>50.00 μM);	-

    • HSV-2:

      Target Organism	Activity
      inhibition of HSV-2 replication in Vero cells(IC50>50.00 μM μM,IC90>50.00 μM);	-

    • SARS-CoV-2:

      Target Organism	Activity
      inhibition of replication in Vero cells(IC50>50.00 μM μM,IC90>50.00 μM);	-

    • HPIV-3: inhibition of replication in Vero cells(IC50>50.00 μM,IC90>50.00 μM);
    • HCoV-229E: inhibition of replication in Vero cells(IC50>50.00 μM,IC90>50.00 μM);
    • HCoV-OC43: inhibition of replication in Vero cells(IC50>50.00 μM,IC90>50.00 μM).

• • Hemolytic Activity

  • • [Ref.35216177]<20% hemolysis against human erythrocytes at concentrations equal or above 25 μM.

• • Cytotoxicity

  • • [Ref.35216177]Vero cells:CC50>100.00 μM

• • Binding Target

  • membrane

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • The 'x' at position 10 is D-hydroxyproline (Hyp).

• • Stereochemistry

  • Mixed(D-Leu9,D-Hyp10)

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP30318.

DRAMP30318

• • Mechanism

  • TL peptides may be able to selectively induce pore formation in highly curved membrane structures (below ~250 nm in diameter), resulting in membrane lysis once a critical number of pores is formed, with the consequence of viral infectivity reduction.

• ·Literature 1

• • Title

  • Broad-Spectrum Antiviral Activity of the Amphibian Antimicrobial Peptide Temporin L and Its Analogs.

• • Pubmed ID

  • 35216177

• • Reference

  • Int J Mol Sci. 2022 Feb 13;23(4):2060.

• • Author

  • Zannella C, Chianese A, Palomba L, Marcocci ME, Bellavita R, Merlino F, Grieco P, Folliero V, De Filippis A, Mangoni M, Nencioni L, Franci G, Galdiero M.